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. 2018 Jul 3;8:10048. doi: 10.1038/s41598-018-28443-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CAP-induced senescence and Ca²⁺ influx are linked to each other. (A,B) Mel Im (A, n = 389) and Mel Juso (B, n = 660) were simultaneously loaded with the Ca²⁺ chelator BAPTA AM (10 µM) and fura-2 AM (3 µM) and cytoplasmic Ca²⁺ was measured during and after 30 s CAP exposure. (C,D) Senescence-associated ß-galactosidase staining of Mel Juso (n = 20, each from four independent runs) 48 h after CAP exposure for 30 s or 60 s and with or without BAPTA AM (10 µM) pretreatment. (C) Representative pictures were taken with a 20 × magnification in bright field illumination. Senescent cells showed a blue staining due to senescence-associated ß-galactosidase activity. Traces are mean and 99% confidence interval (A,B) and bars are mean ± SEM (D).