Table 1.
Capsular typing of 96 human-isolated C. canimorsus strains from the CCUG collection
| Strain | PCR typing | WB typing | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ABC | A | B | C | D | E | Serovar | A | B | C | D | E | F | G | H | I | Serovar | |
| G01 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G02 | + | + | − | + | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G03 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G04 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G06 | − | − | − | − | − | − | nt | − | − | − | − | − | − | − | − | − | nt |
| G07 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G09 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G11 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G12 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G16 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G18 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G20 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G22 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G23 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G24 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G26 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G27 | − | − | − | − | + | − | D | nd | nd | nd | + | nd | nd | nd | nd | nd | D |
| G28 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G29 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G30 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G31 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G33 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G34 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G35 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G37 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G38 | + | + | − | + | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G39 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G40 | + | + | − | + | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G41 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G43 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G44 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G45 | − | − | − | − | − | − | nt | − | − | − | − | − | − | − | − | − | nt |
| G46 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G47 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G48 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G49 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G50 | + | − | − | + | − | − | C | nd | nd | nd | nd | nd | nd | nd | nd | nd | C |
| G51 | + | − | + | − | − | − | B | nd | + | + | nd | nd | nd | nd | nd | nd | B |
| G52 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G54 | + | + | − | + | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G56 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G57 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G58 | + | − | + | − | − | − | B | − | − | − | − | − | − | − | − | − | nt |
| G59 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G60 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G61 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G63 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G64 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G65 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G66 | − | − | − | − | + | − | D | nd | nd | nd | + | nd | nd | nd | nd | nd | D |
| G67 | + | + | − | + | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G68 | + | + | − | + | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G69 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G70 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G71 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G72 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G73 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G75 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G76 | + | + | − | + | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G79 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G80 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G81 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G82 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G83 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G85 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G86 | − | − | − | − | + | − | D | nd | nd | nd | + | nd | nd | nd | nd | nd | D |
| G88 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G90 | − | − | − | − | + | − | D | nd | nd | nd | + | nd | nd | nd | nd | nd | D |
| G91 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G92 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G93 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G94 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G95 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G96 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G97 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G98 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G99 | − | − | − | − | + | − | D | nd | nd | nd | + | nd | nd | nd | nd | nd | D |
| G100 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G101 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G102 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G103 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G104 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G106 | − | − | − | − | + | − | D | nd | nd | nd | + | nd | nd | nd | nd | nd | D |
| G108 | + | + | − | + | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G109 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G110 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G112 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G113 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G114 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G115 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G116 | + | + | + | − | − | − | A | + | nd | nd | nd | nd | nd | nd | nd | nd | A |
| G117 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G118 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G119 | + | − | − | + | − | − | C | nd | nd | + | nd | nd | nd | nd | nd | nd | C |
| G120 | + | − | + | − | − | − | B | nd | + | nd | nd | nd | nd | nd | nd | nd | B |
| G121 | − | − | − | − | + | − | D | nd | nd | nd | + | nd | nd | nd | nd | nd | D |
Capsular typing was determined by PCR using the oligonucleotides given in Supplementary Table S2. The results were interpreted as previously described14: strains positive for PCR A, PCR A and PCR B or PCR A and PCR C were typed as A, strains positive for only PCR B were typed as B, and strains positive for only PCR C as C. Western blot analysis on polysaccharidic structures was performed using specific polyclonal rabbit antisera. nd not determined, nt non-typable