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. 2018 Jul 3;8:10004. doi: 10.1038/s41598-018-28296-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spermine enhances colocalisation of Tubb6 with PrP^Sc aggregates in aggresomes. SMB.s15 cells were treated with10 μM CQ or treated with 5 μM of spermine and 10 μM CQ for 24 hrs. (a) Representative immunofluorescence images of spermine + CQ treated cells showing cells with aggresomes stained with antibodies for Tubb6 and PrP^Sc aggregates (lower lane) compared to untreated control cells + CQ (upper lane) (N = 3). (b) Representative immunofluorescence image showing colocalisation of HDAC6 and PrP^Sc aggregates (N = 3). (c) Significantly increased number of cells per microscopic area showing aggresomes on spermine + CQ treatment when compared to untreated control cells+ CQ, each microscopic area contained 5–15 cells, total of 10 microscopic areas were counted. Scale bar = 10 μm. Abbreviations used Chloroquine (CQ).