Figure 6.

Structural centrosome aberrations caused by excess NLP or excess CEP131 are distinct. (a) Representative immunofluorescence images of MDCK cells cultured in 2D monolayers and stained for α-tubulin (red) and DNA (blue). Cells were not induced (no Dox) or induced to express GFP-NLP (green) or GFP-CEP131 (green) for 48 h. Scale bars = 10 µm. (b) MDCK cells were treated as in (a) and stained for γ-tubulin (red). Note that both GFP-NLP and GPF-CEP131 assemble at centrosomes (green), but only excess GFP-NLP also causes the accumulation of γ-tubulin. DNA was stained with DAPI (blue). Scale bars = 10 µm. (c) MDCK cells were treated as in (a) and stained for detyrosinated α-tubulin (red). Note that excess GFP-NLP (green) causes MT stabilization, as revealed by increased staining for detyrosinated α-tubulin, but excess GFP-CEP131 does not. DNA was stained with DAPI (blue). Scale bars = 10 µm.