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. 2018 Jul 3;9(7):740. doi: 10.1038/s41419-018-0756-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Schematic diagram showing the conserved RING and NHL domains in Tn. The B-box and coiled-coil domains are predicted, but poorly conserved. b–f Confocal Z-stack micrographs of abdominal muscles at 24 h APF stained for F-actin (green). b DEOM1 underwent histolysis (*) in the mef2-Gal4/+ control genotype. c DEOM1 histolysis is incomplete (yellow solid line) in mef2>tn RNAi animals. d–f Expression of Tn FL (d) or TnΔNHL (f) restores histolysis, while removal of the RING domain completely blocks DEOM degeneration, indicating this region is essential for normal histolysis (e). g Bar graph showing a significant decrease in DEOM histolysis in mef2>tn RNAi; tnΔRING flies. h–l Confocal Z-stack merged images of DEOM1 (white dotted line) at 12 h APF co-stained for F-actin (red) and DIAP1 (green). Low DIAP1 levels are observed in genotypes in which DEOM histolysis proceeds normally, including mef2-Gal4 (h), mef2>tn RNAi+tn FL (j), and mef2>tn RNAi+tnΔNHL (l). Increased DIAP1 levels are observed in mef2>tn RNAi (i) or mef2>tn RNAi+tnΔRING (k) DEOMs. m Quantification of relative fluorescence intensity levels reveals significantly higher DIAP1 levels in mef2>tn RNAi+tnΔRING muscles. Mean ± SEM (n.s., not significant, ****p < 0.001, **p < 0.01). Scale bar, 100 µm (b–f) and 50 µm (h–l)