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. 2018 Jul 3;9(7):740. doi: 10.1038/s41419-018-0756-x

Fig. 7. Loss of Tn affects DIAPI protein levels and Dronc activation.

Fig. 7

a Total protein extracted from pupae at 0 or 8 h APF was subjected to western blotting to assess DIAP1 or ATP5α protein levels. DIAP1 levels at 8 h APF are increased in tn mutants. b Densitometry quantification of the relative DIAP1 protein levels using ATP5α as a loading control reveals a significant increase in DIAP1 protein at 8 h upon loss of Tn (n = 3). c Dronc processing in WT or tn−/− pupae. Dronc FL (~55 kDa) is present at 24 h APF in WT samples. The cleaved Pr1 form (~40 kDa) is weakly observed during WT metamorphosis (black asterisk), while the active Pr2 cleavage product of Dronc (~37 kDa) predominates from 0 to 24 h APF. Only the Pr1 form is present upon loss of Tn throughout DEOM histolysis. Mean ± SEM (n.s., not significant, ***p < 0.005)