Table 2.
Regulation by PRX of genes encoding blood brain barrier proteins.
| Probe set | Gene | Descriptive Name | Average PRX/control | Protein type | Number of PDZs |
|---|---|---|---|---|---|
| 16932321 | CLDN5 | claudin-5 | 1.48 | TM | |
| 16712854 | MPP7 | Membrane Palmitoylated Protein 7 | 1.38 | Adaptor | 1 |
| 16801400 | CGNL1 | Cingulin Like 1 | 1.31 | Adaptor | |
| 16695490 | F11R | Junctional adhesion molecule A | 1.3 | TM | |
| 16668919 | MAGI3 | Membrane-Associated Guanylate Kinase | 1.28 | Adaptor | 6 |
| 16913315 | DLGAP4 | DLG Associated Protein 4 | 1.25 | Adaptor | |
| 16985704 | OCLN | occludin | 1.24 | TM | |
| 16819794 | CDH5 | Ve-cadherin | 1.24 | TM | |
| 16745693 | ESAM | Endothelial Cell Adhesion Molecule | 1.23 | TM | 1 |
| 16985688 | MARVELD2 | MARVEL Domain Containing 2 | 1.2 | TM | |
| 17082263 | SCRIB | Scribbled Planar Cell Polarity Protein | 1.19 | Adaptor | |
| 16746591 | WNK1 | Serine/threonine-protein kinase WNK1 | 1.18 | Adaptor | |
| 17009008 | TJP1 | Zonula occludens protein 1 (ZO-1) | 1.13 | Adaptor | |
| 17115692 | MPP1 | Membrane Palmitoylated Protein 1 | 1.1 | LA | 1 |
| 16889845 | PARD3B | Par-3 Family Cell Polarity Regulator Beta | 1.07 | Adaptor | 3 |
| 17048188 | CLDN12 | claudin-12 | 1.05 | TM | |
| 16856159 | PARD6G | Par-6 Family Cell Polarity Regulator Gamma | 1.04 | Adaptor | 1 |
| 16806467 | TJP1 | Zonula occludens protein 1 (ZO-1) | 1.04 | Adaptor | 3 |
| 16730268 | AMOTL1 | Angiomotin Like 1 | 1.04 | Adaptor | |
| 16963455 | DLG1 | Discs Large MAGUK Scaffold Protein 1 | 1 | Adaptor | |
| 17085685 | TJP2 | Zonula occludens protein 2 (ZO-2) | 0.99 | Adaptor | 3 |
| 16825154 | ARHGAP17 | Rho GTPase Activating Protein 17 | 0.97 | Adaptor | |
| 16921832 | JAM2 | Vascular Endothelial Junction-Associated Molecule | 0.97 | TM | |
| 17048167 | CLDN12 | claudin-12 | 0.97 | TM | |
| 16785606 | MPP5 | Membrane Palmitoylated Protein 5 | 0.97 | Adaptor | 1 |
| 16712825 | MPP7 | Membrane Palmitoylated Protein 7 | 0.95 | Adaptor | 1 |
| 17113418 | AMOT | Angiomotin | 0.95 | Adaptor | |
| 16736847 | LIN7C | Protein lin-7 homolog C | 0.94 | Adaptor | 1 |
| 17048165 | CLDN12 | claudin-12 | 0.92 | TM | |
| 16959441 | AMOTL2 | Angiomotin Like 2 | 0.85 | Adaptor | |
| 16713230 | PARD3 | Partitioning defective 3 homolog | 0.76 | Adaptor | 3 |
Level of PRX regulation is shown for elected genes that encode proteins implicated by Daneman et al. (Daneman et al. 2010) to participate in BBB function. We extracted averaged values of PRX/RFP expression levels from the same dataset from human cerebral endothelial cells used to generate Table 1. Protein type and the presence of PDZ domains in each protein was extracted from uniprot.org. CLDN12 and MPP7 are listed more than once since they have more than one probe set on the microarray. JAM4 and ASHL1 were not represented. TM = Transmembrane. LA = Lipid Anchored.