Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 3;9:2592. doi: 10.1038/s41467-018-05008-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — Increased levels of DNA damage in SAN1−/− cells exposed to MMC. a Immunoblots of γH2AX from HeLa WT and SAN1−/− cells treated for various times with vehicle or 0.045 μM MMC. GAPDH was a loading control. b Quantification of immunoblot time course (N = 3). Statistical significance determined by t-test with Welch’s correction. c Immunofluorescence (IF) staining of γH2AX and DNA (Draq5) 30 hrs after treatment with vehicle or 1 μM MMC. d Quantification of γH2AX intensity in vehicle and MMC treated HeLa WT and SAN1−/− cells. Statistical significance determined by t-test with Welch’s correction (N = 3 biological replicates, at least 250 cells per sample were analyzed). e IF staining of 53BP1 and DNA (Draq5) 30 h post-treatment with vehicle or 1 μM MMC. f Quantification of percentage of cells with >10 53BP1 foci in HeLa WT and SAN1−/− cells. Statistical significance determined by unpaired t-test (N = 3 biological replicates, at least 200 cells per sample were analyzed)