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. 2018 Jul 3;8:10001. doi: 10.1038/s41598-018-28292-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of pregnancy on fecal microbiota characteristics. Shannon diversity (A), richness (B) and the firmicutes/bacteroidetes ratio (C) in the fecal microbiota of pregnant and non-pregnant BALB/c and B6 (n = 5). Results are shown as dot plots + means and were tested for overall strain and pregnancy effects using a Two-way ANOVA, followed by a Bonferroni post-hoc test to test for strain specific pregnancy effects when interaction was found. Significant pregnancy effects are indicated with dashed lines (p < 0.05). RDA plot (D) showing the variation explained by the components genotype and pregnancy (n = 5). The total variation that can be explained by the variables genotype (14.3%) and pregnancy (12.5%) is 26.8%. Both variables are significant in explaining the variation (Monte Carlo permutation, p < 0.05).