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. 2018 Jul 3;8:9990. doi: 10.1038/s41598-018-28245-9

Figure 2.

Figure 2

Monitoring of actin polymerization using pyrene actin. (A and B) Pyrene-labeled actin solution was polymerized by adding F-actin buffer at the time point of 0, and the increase in the fluorescence of pyrene was observed by using GloMax-20/20 with Luminometer Fluorescent Modules UV (EX: 365–395 nm, EM: 440–470 nm) with or without irradiation with THz waves (w/THz or w/o THz, respectively). The fluorescent signal was measured every 5 min for 20 min at 25 °C. In panel C, the fluorescence was monitored without adding the F-actin buffer. The initial G-actin concentration was 1.2 μM (A and C), or 0.8 μM (B). The relative fluorescence of pyrene at 0 min was defined as 1.0. Data shown are the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01.