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Microscopic analysis of patients' samples. A: Schematic demonstrating whole tissue analysis. All nerves within 2 mm from the tumor were considered for data collection. B-D: Representation of three different microscopic aspects of PNI observed by H&E, Cytokeratin and S100. B: tumor cells infiltrating the nerve. C: Total encirclement of the nerve by tumor cells. D: At least 33% encirclement of the nerve. Arrows point to tumor cells, asterisks indicate nerves. Scale bars = 500 μm.
