Figure 2.

Histones lost from LPS-challenged bone marrow-derived macrophages (BMDMs) are not degraded. (A) The total content of the culture well, comprising both BMDMs and their supernatant, was lysed in hot SDS-PAGE loading buffer, and histone H3 was quantitated by western blotting. Significance was assessed with a t-test. This experiment was repeated four times. (B) Histone H3 quantitation in BMDMs after LPS challenge, in the presence or absence of the proteasome inhibitor MG132. H3 quantitation and representation of the results as in Figure 1. One-way ANOVA with Tukey’s posttest; *p < 0.05; **p < 0.01.