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. 2018 Jun 27;9:1406. doi: 10.3389/fimmu.2018.01406

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Copyright © 2018 Keizer, Kamp, van Mierlo, Kuijpers and Wouters.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Size-exclusion chromatography (SEC) of mannan-binding lectin (MBL)-deficient and MBL-sufficient serum. SEC of MBL-deficient serum (A,C) or MBL-sufficient serum (B,D). Serum was fractionated on a Superdex™ 200 10/300 GL column (A,C). Fractions were tested for the presence of MBL, or MBL–MBL-associated serine protease (MASP)-1/3, MBL–MASP-2 complexes on mannan-coated plates and compared with a pool of normal human sera (NHS). (B,D) Mannan-coated plates were pre-incubated with fixed amount of recombinant MBL, and followed by the different fractions of MBL-deficient or MBL-sufficient serum, and the formation of new MBL–MASP-1/3 or MBL–MASP-2 complexes was detected and compared with a pool of NHS.