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. 2018 Jun 27;9:1406. doi: 10.3389/fimmu.2018.01406

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Copyright © 2018 Keizer, Kamp, van Mierlo, Kuijpers and Wouters.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Size-exclusion chromatography (SEC) of mannan-binding lectin (MBL)-reconstituted MBL-deficient serum. SEC of MBL-deficient serum reconstituted to 50 µg/ml MBL. (A) Fractions were tested for the presence of MBL–MBL-associated serine protease (MASP)-1/3 and MBL–MASP-2 complexes on mannan-coated plates. (B) Mannan-coated plates were pre-incubated with MBL, and followed by the different fractions of reconstituted MBL-deficient serum, and the formation of MBL–MASP-1/3 and MBL–MASP-2 complexes was detected.