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. 2018 Jun 27;9:1494. doi: 10.3389/fimmu.2018.01494

Figure 5.

Figure 5

Effect of macrophage migration inhibitory factor (MIF) neutralization in the expression of cytokines from primary human immunodeficiency virus-infected and uninfected monocyte-derived macrophages (MDMs). Serial dilutions of a neutralizing αMIF antibody (clone NIHlllD.9) (A), and the MIF antagonist MIF098 (B) were used to inhibit MIF activity in infected (In, red lines) and uninfected (Un, gray lines) MDMs at a constant concentration of this cytokine (25 ng/ml). Data represent three independent experiments (donors), each evaluated in duplicate. Data represent the mean ± SD. Data were analyzed by two-way ANOVA followed by Tukey’s post-test (intragroup analysis, In group only; ****p < 0.0001) or by Sidak’s post-test (intergroup, In versus Un; #1p < 0.05, #2p < 0.01, #3p < 0.001, #4p < 0.0001). Asterisks corresponding to the intragroup analysis are shown above the horizontal bars, and those from the intergroup analysis are shown above points corresponding to each antagonist or antibody dilution.