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. 2018 Jun 27;9:1494. doi: 10.3389/fimmu.2018.01494

Figure 8.

Figure 8

Infection of monocyte-derived macrophages (MDMs) and unactivated CD4+ T-cells with T/F viruses reproduce the results obtained with the R5-tropic (BAL) and the X4-tropic (NL4-3) laboratory strains. (A) Expression of IL-1β, IL-8, IL-6, and IL-10 in supernatants from uninfected (Un) and R5-tropic T/F-infected (In) MDMs. Data represent mean ± SD from three independent donors. Data were analyzed by two-way ANOVA followed by Tukey’s post-test. *p < 0.05, **p < 0.01, ****p < 0.0001. (B) Human immunodeficiency virus (HIV) p24 antigen production from primary unactivated CD4+ T-cells incubated with supernatants from uninfected and infected MDMs treated with 1 ng/ml (left panel) or 25 ng/ml (right panel) macrophage migration inhibitory factor (MIF). Treated CD4+ T-cells were infected with a dual-tropic T/F virus and viral production was evaluated at 4 and 7 days post-infection. Data represent mean ± SD from five independent donors. Data were analyzed by two-way ANOVA followed by Sidak’s post-test. *p < 0.05.