Fig. 5. RNAseq reveals potential downstream signaling of u50535.

a Heat map displays altered expressions of 333 genes (≥1.5-fold change) in si-NC-treated and siRNA-u50535-treated DLD1 cells (details genes in supplementary Table. S2). b Gene ontology (GO) analysis of the altered genes were involved in biological processes (BP) function, which include cell proliferation, cell cycle, apoptosis, adhesion, morphology, immune system and so on. c GSEA reveals enrichment of u50535 associated genes in “HALLMARK INFLAMMTORY RESPONSE”, “HALLMARK G2M CHECKPOINT”, and “MORF ACTG1”. d The altered genes selected from GSEA were confirmed by real-time PCR in knockdown or overexpression of u50535