FIGURE 3.

Multi-compartment expression of GFP in Drosophila cells. (A) Confocal microscopy of cytoplasmic GFP expression in Drosophila S2 cells. A strong GFP signal is observed in the cytoplasm of several CdCl₂-induced cells (green) while it is absent in uninduced cells. DNA was stained with methyl green (blue) (Prieto et al., 2014). Scale bar: 15 μm. (B) Fifteen percent SDS-PAGE of GFP purification from Drosophila culture media with a Strep-TactinXT resin. MM, molecular marker; BC, before column; AC, after column; E, eluted fraction with 50 mM biotin. The purified GFP protein containing the N-terminal twin-Strep-tag (twin-GFP) is indicated with an arrow with the expected molecular mass in parenthesis. Numbers at left of gel corresponds to molecular mass of the marker in kilodalton. A photograph of the eluted twin-GFP protein is shown.