Table 1. Up-regulated proteins and associated pathways: Comparison of response to calcium and Aquamin^®.

Relative abundance (Average 1.8 fold-change or above relative to 0.15 mM calcium of 3 Adenomas)

	Average FC (From 3 Adenomas)
A: Proteins	Calcium	Aquamin®	Aquamin®
	(1.5 mM)	(0.15mM)	(1.5mM)
Proteins common with all 3 Interventions; CA 1.5, AQ 0.15 & AQ 1.5 (2 common proteins)
None significant
Proteins common with 2 Interventions; AQ 0.15 & AQ 1.5 mM (1 common protein)
Merlin (NF2)	1.5±0.1*	1.9±0.3*	2.4±0.7*
Proteins common with 2 Interventions; CA 1.5 & AQ 1.5 mM (9 significant out of 15 common proteins)
Keratin-10 (KRT10)	2.0±0.4*	1.2±0.2	5.3±6.4
Keratin-2 (KRT2)	1.9±0.4*	1.0±0.1	4.6±5.4
Histone H1.5 (HIST1H1B)	1.9±0.4*	1.5±0.8	2.8±1.6
Histone H1.3 (HIST1H1D)	1.8±0.4*	1.5±0.8	2.7±1.5
60S ribosomal protein L7a (RPL7A)	1.8±0.3*	1.6±0.9	2.3±1.4
Histone H1.0 (H1F0)	1.8±0.4*	1.3±0.6	2.3±0.9
Chromatin target of PRMT1 protein (CHTOP)	1.9±0.2*	1.5±0.6	2.1±0.7
Olfactomedin-4 (OLFM4)	2.1±0.6*	1.0±0.0	2.0±0.6*
Brain acid soluble protein 1 (BASP1)	1.8±0.7	0.6±0.1	1.8±0.4*
Up-regulated Proteins; CA 1.5 mM (3 common proteins)
None significant
Up-regulated Proteins; AQ 0.15 mM (1 significant out of 5 common proteins)
ATP-dependent RNA helicase (DDX52)	1.6±0.1*	2.0±0.3*	1.6±0.2*
Up-regulated Proteins; AQ 1.5 mM (6 significant out of 17 common proteins)
keratin, type II cytoskeletal 5 (KRT5)	1.5±0.2*	1.1±0.3	5.8±6.6
Histone H1.2 (HIST1H1C)	1.7±0.3*	1.5±0.8	2.8±1.7
Histone H1.4 (HIST1H1E)	1.7±0.4*	1.1±0.4	2.0±0.6*
Ribonuclease P protein subunit p38 (RPP38)	1.3±0.1*	1.7±0.4*	1.9±0.7
60S ribosomal protein L14 (RPL14)	1.5±0.1*	1.4±0.6	1.8±0.8
Fatty acid-binding protein, liver (FABP1)	1.5±0.2*	1.1±0.0	1.8±0.4*

B. Significant Pathways (Reactome V63)	Entities pValue
Activation of DNA fragmentation factor	1.4×10−11
Apoptosis induced DNA fragmentation	1.4×10−11
Formation of Senescence-Associated Heterochromatin Foci	3.9×10−11
Apoptotic execution phase	1.4×10−8
DNA Damage/Telomere Stress Induced Senescence	3.0×10−8
Cellular Senescence	3.9×10−6
Apoptosis	4.6×10−6
Programmed Cell Death	5.3×10−6
Major pathway of rRNA processing in the nucleolus and cytosol	1.5×10−4
rRNA processing in the nucleus and cytosol	1.9×10−4
rRNA processing	2.2×10−4
Cellular responses to stress	2.8×10−4
Cellular responses to external stimuli	6.3×10−4
Formation of the cornified envelope	9.6×10−4
Metabolism of RNA	2.8×10−3
Keratinization	4.2×10−3
Type I hemidesmosome assembly	0.02
RHO GTPases activate PAKs	0.03

Values represent average fold-change compared to control conditions (0.15 mM calcium) ±standard deviations. Only statistically significant proteins presented in table 1.

^*Represents significance in up-regulation as compared to the control at P < 0.05 level.