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. Author manuscript; available in PMC: 2019 Jun 10.
Published in final edited form as: J Neurochem. 2018 Jun 10;145(6):489–503. doi: 10.1111/jnc.14330

Figure 2. Heat inactivates immunoglobulins detected in α-syn AAb ELISA.

Figure 2

Diluted serum or CSF of two HC and two PD patients were prepared in PBS and heated to 50°C, 90°C, or kept on ice for 10 minutes prior to addition to ELISA. Activity on ice was similar to activity following incubation at 50°C for 10 minutes. In contrast, incubation at 90°C for 10 minutes denatured the immunoglobulins in the sample and ELISA activity was lost. Each column represents mean ± standard deviation of three technical replicates (n=3 wells). HC = healthy control; PD = Parkinson’s disease.