Table 2.

Data from All Samples Collected and Processed in This Study.^a

No.	Patient History	Surgical Findings	OCT	EUB335	NTHi		SP		Mcat		PM		Notes
1	ETD, RAOM, COME	MPE	Biofilm	+	+	Δ			+		+
2	ETD, CMOM	MPE	Biofilm	+	+	Δ
3	ETD, CMOM	Bulging TM, MPE	Biofilm	+		Δ		Δ		Δ		Δ
4	ETD, CMOM	Injected, dull TM, MPE	Biofilm	+		Δ	+		+	Δ	+	Δ
5	ETD, CMOM	Bulging, injected TM, MPE	Biofilm	+		Δ
6	ETD, CMOM	Bulging, injected TM, ME	Biofilm	+	+	Δ		Δ				Δ
7	ETD, RAOM	Retracted (mild) TM, SE	Biofilm	+	+		+			Δ	+
8	ETD, CMOM	Injected, dull TM, thick MPE	Biofilm	+	+		+		+		+		PCR (NEG)
9	ETD, CMOM	Injected, dull TM, thick MPE	Biofilm	+	+	Δ			+		+
10	ETD, RAOM, past TT (R)	ME	Biofilm					Δ					Poor-quality FISH
11	ETD, CMOM	Retracted (mild), injected TM, thick ME	Biofilm	+	+		+				+		No PCR
12	ETD, CMOM, past TT (Ex)	Retracted (mild) TM, SE	Biofilm	+									PCR (NEG)
13	ETD, RAOM, COME	ME	No OCT	+	+					Δ
14	ETD, CMOM	Retracted (mild), injected TM, thick ME	Biofilm	+	+				+		+		PCR (NEG)
15	ETD, CMOM	Retracted (mild), injected TM, thick ME	Biofilm	+	+	Δ	+	Δ	+	Δ	+	Δ
16	RAOM, OME, SL	ME	Biofilm	+		Δ		Δ				Δ
17	ETD, RAOM	ME	Biofilm	+	+	Δ	+		+		+
18	ETD, OME, past TT (R)	ME, tonsillectomy + adenoidectomy	Biofilm	+	+		+		+		+		PCR (NEG)
19	ETD, COME	ME	Biofilm	+					+				PCR (NEG)
20	Chronic ETD, COME, torticollis	ME	No OCT	+			+		+		+		No PCR
21	Chronic ETD, COME, torticollis	ME	Biofilm	+	+		+		+	Δ	+
22	ETD, COME, SL	ME	Biofilm	+	+		+	Δ	+	Δ	+	Δ
23	ETD, COME, SL	ME	Biofilm	+	+		+	Δ	+	Δ	+	Δ
24	ETD, RAOM	Injected, “full” TM, glue-like effusion	Biofilm	+			+		+		+		PCR (NEG)
25	ETD, RAOM	Injected, “full” TM, glue-like effusion	Biofilm	+	+	Δ	+				+
26	ETD, CMOM, frequent OM, (DS)	Retracted (mild) TM, thick ME	Biofilm	+	+								PCR (NEG)
27	ETD, CMOM, conductive hearing loss, SL	Thick ME	Biofilm	+	+		+				+		PCR (NEG)
28	ETD, CMOM, conductive hearing loss, SL	Thick ME	Biofilm			Δ	+		+		+
29	ETD, CMOM, persistent OME	Injected, dull TM, thick ME	Biofilm	+	+		+				+		PCR (NEG)
30	ETD, CMOM, persistent OME	Injected, dull TM, thick ME	Biofilm	+	+		+		+		+		PCR (NEG)
31	ETD, CMOM, persistent OME	Injected, dull TM, thick ME	Biofilm	+		Δ	+		+		+
32	ETD, CMOM, persistent OME	Injected, dull TM, thick ME	Biofilm	+	+	Δ	+				+
33	ETD, CMOM, past TT (Ex)	Retracted (severe), thin TM, thick ME	Biofilm	+	+		+		+		+		PCR (NEG)
34	ETD, CSOM, persistent OME, OSA tonsillar and adenoid hypertrophy (3+)	Thick SE	Biofilm	+	+		+		+		+		No PCR

Abbreviations: CMOM, chronic mucoid otitis media; COME, chronic otitis media with effusion; CSOM, chronic serous otitis media; DS, Down syndrome; ETD, eustachian tube dysfunction; Ex, extruded; FISH, fluorescence in situ hybridization; Mcat, Moraxella catarrhalis; ME, mucoid effusion; MPE, mucopurulent effusion; NEG, negative; NTHi, Haemophilus influenzae; OCT, optical coherence tomography; OME, otitis media with effusion; OSA, obstructive sleep apnea; PCR, polymerase chain reaction; PM, polymicrobial; R, resolved (extruded and healed); RAOM, recurrent acute otitis media; SE, serous effusion; SL, hearing or speech and language delay concerns; SP, Streptococcus pneumoniae; TM, tympanic membrane; TT, tympanostomy tube.

^aPositive identification of a biofilm or bacterial strain as OCT is marked as “biofilm,” FISH as +, and PCR as Δ for each genetic probe.