Figure 5.

Active Sampling and Associated Response Changes Are Dynamically Linked to Task Engagement

For all panels, green, task engaged (Eng.); black, task disengaged (Dis.).

(A) Experimental paradigm for switches in task engagement.

(B) Left: lick raster across task switches for CS⁺ and CS⁻ stimuli for an example mouse. Blue-shaded region shows 1 s response window after odor offset. Right: plot showing changes in anticipatory lick rate between engaged and disengaged trials for the CS⁺ for each mouse.

(C) Top: plot showing MID across trials for an example cell-odor pair. Middle: corresponding plot to show odor FR response for the same cell-odor pair across trials. Bottom: corresponding plot to show V_m responses (after spike subtraction) across trials. Traces on the right show example nasal flow and V_m traces for trials corresponding to those indicated by dotted lines on the left plots.

(D) Example traces for a different cell-odor pair during task engagement (top trace, trial #14), disengagement (middle trace, trial #22), and re-engagement (bottom trace, trial #23). Note that response valence can change within a single trial depending on task engagement.

(E) For all eight cell-odor pairs, changes in MID between task engagement, disengagement, and re-engagement (asterisks denote result of paired t tests). Error bars show SD.

(F) As for (E), but for changes in 2 s FR responses.

(G) As for (E), but for changes in 500 ms V_m responses.

(H) Scatterplot of MID versus V_m response across trials for an example cell-odor pair.

(I) Left: boxplots to show R² values (as for example in H) for all six FR responses showing significant changes between engagement shifts, alongside those for shuffled data. Right: as for left, but for V_m responses.