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. Author manuscript; available in PMC: 2018 Aug 19.
Published in final edited form as: Mucosal Immunol. 2018 Feb 19;11(4):1158–1167. doi: 10.1038/s41385-018-0009-4

Figure 4.

Figure 4

Rectal infection with LCMV results in recruitment and activation of immune cells to the colonic lamina propria and enhances systemic lymphocyte migration. Male WT mice were i.rec. infected with LCMV, and at indicated time points, the frequency and activation of indicated immune cell populations in the lamina propria, iLN and spleen was determined by flow cytometry. (a) Representative gating strategy depicting a LP sample at day 3 p.i.. Lin: TCRβ/CD19/NK1.1/CD326 (EpCam). (b) Number of Ly6c+ monocytes and dendritic cells (DCs) in the LP. (c) Median fluorescence intensity (MFI) of CD86 on DCs and Ly6c+ monocytes. Absolute counts of various lymphocytes in the lamina propria of the colon (d), iLN (e), and spleen (f). B cells: TCRβ- CD19+; CD8 T: TCRβ+ CD8+; CD4 T: TCRβ+ CD4+. Data represented from one of three independent experiments (b, c), or were pooled from two independent experiments (d-f). n = 3 (b, c) or 6 (d-f) per time point. Error bars represent mean ± SEM. UI, uninfected.