Fig. 5.

Neurons express LRP1 and bind hemopexin. (A-D) Phase contrast and fluorescence images of fixed cultures immunostained with anti-NeuN and anti-LRP1. (E-H) Fixed cultures stained with anti-NeuN and 80 μg/ml solution of Alexa Fluor 568-labeled Hpx for 4 h; the labeled Hpx apoform was used since hemin binding quenched fluorescence when applied to fixed cells. (I, J) Viable culture incubated with 1 μM Alexa Fluor 568-labeled Hpx-hemin solution for 24 h. Uptake by phase bright neuron somata is apparent.