Scatter-plot showing number of BDNF positive cells in different areas of the adult zebrafish brain. Note that alcohol (0%, control, white; or 1% alcohol black) was delivered during embryonic development and immunostaining to detect BDNF positive cells was conducted in adult zebrafish. Each point represents results from a single subject. The different brain areas analyzed were: lateral zone of the dorsal telencephalic area (Dl), medial zone of the dorsal telencephalic area (Dm), dorsal nucleus of the ventral telencephalic area (Vd), ventral nucleus of the ventral telencephalic area (Vv), parvocellular preoptic nucleus (PPa), ventral habenular nucleus (Hav), corpus cerebelli (CCe) and inferior reticular formation (IRF). n=6, and 4 sections were used per animal.