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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Mol Cancer Res. 2018 May 2;16(7):1161–1171. doi: 10.1158/1541-7786.MCR-18-0042

Figure 4. Decreased expression of ALDH1A3 in USP22 knockdown tumorsphere cells.

Figure 4

A. Quantitative reverse transcription PCR (qRT-PCR) and Western Blot analysis show both mRNA and protein of ALDH1A3 were downregulated in lung cancer cell line A549 upon USP22 knockdown. B. Western Blot analysis of co-expression of ALDH1A3 and USP22 in CD133+/CD133− cancer cells. C. Western Blot analysis of co-expression of ALDH1A3 and USP22 in CD44+/CD44− cancer cells. D. qRT-PCR and Western Blot analysis show both mRNA and protein of ALDH1A3 were decreased in USP22-KD tumorspheres cells, indicating USP22 might also modulate ALDH1A3 in tumorsphere cells.