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. Author manuscript; available in PMC: 2019 Jul 3.
Published in final edited form as: Biochemistry. 2018 May 23;57(26):3883–3893. doi: 10.1021/acs.biochem.8b00271

Scheme 1.

Scheme 1

Magnetic bioreactor beads in an 96-well plate followed by LC-MS/MS to determine sequence specific on 32 bp oligonucleotide that represents p53 gene fragment, damage sites and product amounts: (A) magnetic beads coated with microsomal cyt P450 enzymes coupled to NADPH regeneration in 96 well filter plate to convert test chemicals to metabolites that can react with 32 bp oligonucleotide; B) Solutions of reacted 32 bp oligonucleotide were separated from magnetic beads, and extracted to isolate the oligonucleotides; C) restriction enzymes cut 32 bp oligonucleotide into smaller fragments; (D) purification of 32 bp oligonucleotide to remove enzymes and salts to prepare the cut 32 bp oligonucleotide for, (E) LC-MS/MS sequence analysis. Center panel shows 96-well plate with a possible experimental reaction plan.