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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Cancer Immunol Res. 2018 May 21;6(7):766–775. doi: 10.1158/2326-6066.CIR-17-0498

Figure 4. Blocking IL1R8 and PD-1 abrogates Treg-mediated suppression of canonical NK cells.

Figure 4

A) Canonical and B) adaptive NK cells cultured with IL2 (50 IU/ml) and APCs ± Tregs (Treg:NK, 1:2) in the presence of blocking antibodies against IL1R8, PD-1, TIM3, or control IgG for 6 days. NK-cell cultures were then stimulated with anti-CD16 antibody, and IL12, and IL18 for 6 hours prior analysis of IFNγ production and proliferation (Ki67). Cumulative data (n = 7) are shown from three independent experiments as mean ± SEM. C) Canonical NK-cell TIM3 (upper panel) and PD-1 (lower panel) expression were analyzed following culture with APCs ± Tregs and in the presence of blocking antibodies against IL37, IL1R8, and PD-1 or IgG. D) Canonical NK cells in culture with APCs were treated with recombinant IL37 (3 μg/ml) or PD-L1 Fc chimera protein (10 μg/ml) and evaluated for the expression of TIM3 and PD-1 expression after 6 days of culture. Cumulative data (n = 6–8) are shown from three independent experiments as mean ± SEM and statistical analyses were performed using pairwise comparison mixed-effects model.