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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Cancer Immunol Res. 2018 May 16;6(7):776–787. doi: 10.1158/2326-6066.CIR-17-0649

Figure 2. Design and purification of CS1-NKG2D biAb by metal-affinity chromatography.

Figure 2

(A) Schematic diagram of the lentiviral construct for mammalian expression of CS1-NKG2D biAb in CHO-S cells. (B) A typical profile of the protein eluted from immobilized metal-affinity chromatography column using stepwise imidazole gradient. (C) SDS-PAGE for eluted protein. Lane 1: molecular weight marker (kDa); Lane 2: protein lysate from mock transduced CHO-S cells; Lane 3: eluted control biAb (dashed arrow); Lane 4: eluted CS1-NKG2D biAb (solid arrow). Results shown are representative of at least ten independent experiments.