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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Mol Cancer Res. 2018 Apr 18;16(7):1077–1091. doi: 10.1158/1541-7786.MCR-17-0685

Figure 2. Bortezomib induces ER stress and autophagy.

Figure 2

(A, B) LS174T (A) and HCT116 (B) cells were treated with mitomycin C and bortezomib for 24 h; BiP/R-BiP, Chop, ATF4, and LC3 were detected with immunoblotting. (C) After treatment, LC3 puncta formation was examined using confocal microscopy in HCT116 cells. (D) Quantification of GFP-LC3 puncta formations. Asterisk ** represents a statistically significant difference (P <0.01). (E, F) LS174T (E) and HCT116 (F) cells were treated with different doses (0-5000 nM) of bortezomib for 24 h, PARP, p-eIF-2α, p62, BiP, R-BiP, Chop, ATF4, and LC3 were detected using immunoblotting.