Skip to main content
. Author manuscript; available in PMC: 2018 Sep 7.
Published in final edited form as: Mucosal Immunol. 2018 Mar 7;11(4):1047–1059. doi: 10.1038/s41385-018-0014-7

Figure 2. Expression of PLA2-IIA is specifically induced by P. gingivalis in oral epithelial cells.

Figure 2

Figure 2

(A) Transcriptional activation of PLA2G2A was evaluated by qRT-PCR in OKF6 cells exposed to different multiplicities of infection (MOIs) of P. gingivalis (Pg) for 24 and 48 hours. (B) Protein levels of PLA2-IIA induced by P. gingivalis at different MOIs for 24 and 48 hours were determined in OKF6 cell lysates by ELISA as described in methods. Normalized amounts of PLA2-IIA in cell lysates per 100μg of total protein are shown (C) Protein levels of PLA2-IIA in cell lysates and IL-8 in supernatants induced by different oral bacterial species at MOI= 1:50 for 48 hours in OKF6 cells were determined by ELISA as described in methods. Normalized amounts of PLA2-IIA in cell lysates per 100μg of total protein are shown. The mean ± SD of six replicates from each treatment group from a representative experiment of at least 2 independent experiments are shown. ND=Non-detectable. *p≤0.01 when bacteria treated groups where compared with unstimulated (Mock) cells at 24 or 48 hours. Aa: Aggregatibacter actinomycetemcomitans.