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. Author manuscript; available in PMC: 2018 Sep 7.
Published in final edited form as: Mucosal Immunol. 2018 Mar 7;11(4):1047–1059. doi: 10.1038/s41385-018-0014-7

Figure 5. Gingipains produced by P. gingivalis are involved in Notch-1 activation and PLA2-IIA production in oral epithelial cells.

Figure 5

(A) Notch-1 activation was determined in OKF6 and TIGK cells by measuring Hes-1 mRNA levels induced by P. gingivalis wild type (WT) or the P. gingivalis mutant strain for all gingipains (rgpA-B-kgp-) after 48h of stimulation by RT-qPCR. Fold changes were calculated with respect to gene expression levels in cells that were not challenged with bacteria (B) PLA2-IIA protein levels produced by OKF6 and TIGK cells in response to P. gingivalis-WT and P. gingivalis-mutant for gingipains [MOI=1:50] for 48h were determined by ELISA in cell lysates. Normalized amounts of PLA2-IIA in cell lysates per 100μg of total protein are shown. (C) Determination of IL-8 levels by ELISA in cell supernatants (SNs) from either OKF6 or TIGK cells was performed as a control for the biological activity of the P. gingivalis mutant strain. The mean ± SD of triplicates from each treatment group from a representative experiment are shown. *p≤0.05 when bacteria treated groups where compared with unstimulated (Mock) cells. ND=Non-detectable.