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. Author manuscript; available in PMC: 2018 Sep 7.
Published in final edited form as: Mucosal Immunol. 2018 Mar 7;11(4):1047–1059. doi: 10.1038/s41385-018-0014-7

Figure 6. Release of P. gingivalis-induced PLA2-IIA into the supernatants is enhanced by F. nucleatum.

Figure 6

(A) PLA2-IIA levels were determined by ELISA in cell lysates normalized to total protein (100 μg) and in supernatants from OKF6 cells exposed to other oral bacteria (Fn-F. nucleatum, Sg-S. gordonii at MOI=1:50) for 24h, after priming cells with P. gingivalis [1:50 for 24h]. (B) Determination of IL-8 levels by ELISA in supernatants was performed as control for biological activity of Fn and Sg. The mean ± SD from two independent experiments by duplicate/triplicate are shown. Interrupted line denotes minimal level of PLA2-IIA detection by ELISA (17 pg/ml). *p≤0.01 when bacteria treated groups where compared with unstimulated (Media) cells.