FIG 4 .

GAP45 is not required for egress. (A) First and final frames from a 40-min time-lapse video of ΔGAP45 schizonts undergoing egress. Schizonts from the first frame that subsequently rupture over the course of the video are circled in black (approximately 80% of the total population). Schizonts that do not rupture are circled in white. (B) Quantification of egress in mock-treated (DMSO) and RAP-treated (ΔGAP45) GAP45:loxP parasites. The top plot shows the proportion of schizonts that undergo egress in each 40-min video. The bottom plot shows the time taken for the schizonts in each video to egress. There were no significant differences between the RAP- and mock-treated populations in the efficiency or kinetics of egress. (C) Western blot showing similar levels of the P50 form of SERA5 (which results from egress-associated proteolytic processing of the P126 precursor [27, 58]) released into culture supernatants of DMSO- and RAP-treated GAP45:loxP schizonts following 45 min of egress.