FIG 8 .

Effect of SDS on E. coli O2 and its ETT2 deletion mutant. Bacteria were grown overnight with shaking at 30°C in defined minimal Davis and Mingioli medium (42) supplemented with 0.005% of each amino acid and with 0.4% glycerol as a carbon source. The cultures were diluted 1:25 into fresh growth medium, grown until they reached an OD₆₀₀ of 0.4, and diluted 1:10 into a sterile 96-well plate for a final concentration of 1 to 10% SDS. The plates were then incubated for 4 min at room temperature, and cell degradation was measured at OD₄₀₅ using a BioTek Eon plate reader.