FIG 7 .

Growth of recombinant HPIV-3 CI-1 EGFP and recombinant HPIV-3 CI-1 EGFP bearing mutated HNs in CV-1 and HAE cells. (A) CV-1 cells were infected with 5,000 PFU of the different recombinant viruses (wt CI HN or panel of mutants). Infected cells were observed using an epifluorescence Nikon TS2R-FL inverted microscope 3 days postinfection. (B) Titration of HPIV-3 CI-1 EGFP viruses (wt CI or HN mutants) of either supernatant fluid from CV-1 cells (squares) or virus collected from HAE cells (circles) 1, 3, and 7 days after infection (PFU/ml). The values represent titers performed in triplicate from a representative experiment that has been repeated three times.