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The NDH-2-deficient strain shows a nonrespiratory phenotype. Growth curves and pH of the media at 8 h of culture are shown for the WT (A) and the Δndh (B), Δnox2 (C), and Δndh Δnox2 (D) mutant strains. Cells were grown under respiration permissive (P [white circles and bars]) and nonpermissive (NP [black circles and bars]) conditions. Growth curves are representative of at least 3 independent experiments. pH data are plotted as the average ± SD from three independent experiments.
