FIG 5 .
The Δndh, Δnox2, and ΔcydA mutant strains are attenuated for virulence in heart, kidney, and spleen. Mice were infected with 2 × 106 CFU of S. agalactiae, and bacteria were counted at 24, 48, and 72 h postinfection in heart (A), kidney (B), and spleen (C). Bacterial fitness of mutants is shown as ratio of the log10 number (CFU/gram) of mutant to WT bacteria. *, P < 0.05, **, P < 0.01, and ****, P < 0.0001 (mutant/WT ratio), by Kruskal-Wallis test followed by Dunn’s multiple comparisons. # denotes a median bacterial load (CFU per milliliter) of zero for the group.