FIG 6 .

Hemolytic activity correlates with NADH/NAD⁺ ratios. Cells were grown under respiration permissive (white bars) or nonpermissive (black bars) conditions until the early stationary phase, then washed and incubated with RBCs for 1 h. Dark and light gray bars represent respiration nonpermissive and permissive growth, respectively, with 0.1% Tween 80 added to the media. Data are plotted as the average ± standard error of the mean (SEM) from three independent experiments. *, P < 0.0001 via two-way ANOVA with Dunnet’s posttest compared to the wild type under nonpermissive conditions.