FIG 7 .

Zafirlukast inhibits S. agalactiae respiration. Cells were grown until the early stationary phase (8 h), and the percentage of respiration was calculated after addition of different concentrations of zafirlukast (A). The percentage of respiration corresponds to the difference in oxygen consumption of cells grown under respiration permissive (P) minus nonpermissive (NP) conditions. (B and C) Overnight growth (B) and oxygen consumption (C) of the Δnox2 mutant strain in the absence or presence of 7.5 µM zafirlukast (Z). Data are plotted as the average ± SD from three independent experiments. In panels B and C, *, P < 0.05, **, P < 0.005, and ***, P < 0.001, via two-way ANOVA with Tukey’s posttest for the indicated comparisons. No significant difference was observed between conditions NP, NP+Z, and P+Z.