TABLE 2 .
Bacterial strains and plasmids used in this study
| Strain or plasmid | Main characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| S. agalactiae | ||
| NEM316 | Serotype III isolated from neonatal blood culture | 20 |
| Δndh mutant | NEM316 with ndh gene deleted | This work |
| Δnox2 mutant | NEM316 nox2 ΩaphA-3 Δnox2 Kmr | 23 |
| Δndh Δnox2 mutant | Δnox2 mutant with ndh gene deleted, Kmr | This work |
| NEMJ01 | NEM316 cydA::aphA-3 Kmr | 4 |
| E. coli | ||
| TG1 | Host strain for sequencing | Lucigen |
| TOP10 | Host strain for molecular cloning | Invitrogen |
| c43(DE3) | Host strain for protein overproduction | Avidis, France |
| Plasmids | ||
| pBR322-pGhost8 | Thermosensitive vector, Tetr | 63 |
| pTCV-lac | Shuttle vector, Eryr Kmr | 64 |
| pTCV-ndhC | ndh complementation plasmid | This work |
| pET-22b | Cloning vector, Ampr | Novagen |
a
Ampr, Kmr, Tetr, and Eryr indicate resistance to ampicillin, kanamycin, tetracycline, and erythromycin, respectively.