Figure EV4. ERK and phospho‐ERK levels in the cell body are not affected by axonal Aβ1–42 treatment.
Hippocampal neurons were cultured in microfluidic chambers for 10–11 DIV, and axons were transfected with scrambled or Vim‐targeting siRNA. 24 h after transfection, axons were treated with vehicle or Aβ1–42 for 1 h. ERK and phosphor‐ERK levels were determined by quantitative immunocytochemistry. Mean ± SEM of 24–30 optical fields per condition (n = 3 independently performed experiments per group). No significant differences were detected between DMSO and Aβ1–42 neurons; two‐way ANOVA with Bonferroni's multiple comparisons test.