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. 2001 Oct 12;2(11):research0045.1–research0045.10. doi: 10.1186/gb-2001-2-11-research0045

Figure 6.

Figure 6

Measurement of the configuration of tagged loci in homozygous plants from line EL702C. (a) A stacked image from a guard cell nucleus that contains three GFP spots was created. Scale bar = 5 μm. (b) Outline of nucleus (green wire-frame), position of the nucleolus (blue solid) and GFP fluorescence spots (red solids) were extracted from the stacked images, and a three-dimensional model was created. (c) The three-dimensional model was used to rotate the image by 90° in the y-axis, and the distance between the three GFP spots (a, b and c) was measured. Distance dab = 0.88 μm, dbc= 1.13 μm, dac = 1.58 μm.