Figure EV2. The MSP domain of MOSPD2 is sufficient to interact with STARD3 and STARD3NL .

• A
  Immunoprecipitation experiment between STARD3 (WT and FFAT‐deficient) and endogenous MOSPD2. Proteins were immunoprecipitated using anti‐STARD3 antibodies or control IgG. Protein extracts and immunoprecipitates were analyzed by Western blot using anti‐MOSPD2, anti‐STARD3, and anti‐actin antibodies.
• B, C
  Interaction of STARD3 (B), or STARD3NL (C), with the recombinant MSP domain of MOSPD2. The WT and the RD/LD mutant MSP domain of MOSPD2 were bound to a Ni²⁺‐NTA resin and used in a pull‐down assay with protein extracts of control (HeLa/Ctrl), STARD3, and STARD3 FA/YA FFAT‐defective mutant (B), and STARD3NL and STARD3NL FA/YA FFAT‐defective mutant (C) expressing cells. Total and bound proteins were analyzed by Western blot using anti‐STARD3 (B), anti‐STARD3NL (C), anti‐HIS tag (B and C), and anti‐actin (B and C).

Source data are available online for this figure.