Figure 6. FFAT‐containing proteins recruit the ER‐resident MOSPD2 protein to interorganelle contact sites.

• A
  GFP‐MOSPD2 (green)‐expressing cells were stained with an anti‐Lamp1 antibody (red) to label late endosomes/lysosomes.
• B–F
  GFP‐MOSPD2 (green)‐expressing cells were transfected with Flag‐STARD3NL (B), Flag‐STARD3 (C), Flag‐ORP1L (D), Flag‐STARD11 (E), or HA‐PTPIP51 (F), and labeled using anti‐Flag (B–E; magenta), anti‐HA (F: magenta), and anti‐Lamp1 (B–D; red), GM130 (E; red), or OPA‐1 (F; red) as markers of late endosomes/lysosomes, Golgi, and mitochondria, respectively.

Data information: The subpanels on the right are higher magnification (3.5×) images of the area outlined in white. The Overlay panel shows merged green and magenta images. Scale bars: 10 μm.