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. 2018 Jun 11;19(7):e45543. doi: 10.15252/embr.201745543

Figure 1. Characterization of the Cas1 and Cas2‐DnaQ proteins of the Streptococcus thermophilus DGCC7710 CRISPR4‐Cas system.

Figure 1

  1. Schematic organization of the CRISPR4‐Cas locus. The DnaQ domain is fused to the C‐terminus of the Cas2 protein.
  2. Molecular mass of the St4 Cas1:Cas2‐DnaQ complex obtained by the size‐exclusion chromatography. The estimated molecular mass of the complex is 250 ± 30 kDa, which correlates well with theoretical mass of 220 kDa of the complex. Coomassie blue‐stained sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) of the isolated Cas1:Cas2‐DnaQ complex is shown as an inset.
  3. Mapping of Cas2 determinants required for the Cas1:Cas2‐DnaQ complex formation. Deletion analysis indicates that Cas1 domain and the N‐Cas2 domain (1–124 aa), but not the DnaQ domain (125–301 aa), are required for complex assembly.