Fig. 5.

Analysis of importin 13 cargo candidates enriched with RanQ69L. A, HeLa cells were transfected with plasmids coding for C-terminal HA- or N-terminal GFP-GST-tagged importin 13 cargo candidates (compare Fig. 2C) and FLAG-importin 13 or an empty vector. Expressed proteins were detected by indirect immunofluorescence using anti-HA or anti-FLAG antibodies or directly via the GFP-tag. Scale bars, 20 μm. B, Dot plot of the log2-ratios of nuclear and cytoplasmic intensities of importin 13 cargo candidates with average values (green bars) and standard deviation (error bars). Data were scored from a minimum of three independent experiments. n, number of cells analyzed. ***, p value < 0.001. See Fig. 2C and Table I and S1 for additional proteins tested.