Fig. 6.

Importin 13 cargo candidates interact directly with importin 13. GST- (A) or MBP- (B) tagged importin 13 cargo candidates or the tags alone were immobilized on glutathione or amylose beads, respectively, and incubated with His-importin 13 in the absence or presence of RanQ69L(aa1–180)-GTP or Ubc9, as indicated. Bound proteins were analyzed by SDS-PAGE, followed by Coomassie staining (top) and immunoblotting with anti-importin 13 antibodies (bottom). GST-Ubc9 served as a positive control for importin 13 binding. The input corresponds to 10% or 1% of His-importin 13 used in the binding reactions for the Coomassie gels or the Western blots, respectively.