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Schematic representation of the protein discovery and validation workflow by use of label-free quantitative (LFQ) proteomics and Selected Reaction Monitoring (SRM) analysis. The shotgun proteomics study with case-control design (n = 67) was conducted to dissect comparative saliva proteome along the different disease stages. An independent cohort (n = 82) was used to verify sixty-five candidate markers by SRM. More details can be found under Experimental Procedures.
