Fig. 5.

Comparison of the shared peptides between LCL C1R05 and P50: IR>1.0 subsets. A total of 3098 (C1R05: black bars) and 2262 (P50: white bars) peptides were included in the IR>1.0 subset from each cell line. A, Peptide length distribution, B, Percentage of P(-1) residues with low, intermediate and high susceptibility to ERAP1, C, Percentage of P1 residues with low, intermediate and high susceptibility to ERAP1, D, theoretical affinity of the peptides for B*27:05, as estimated by their IC50 values, E, Hydrolytic activity of recombinant variants of ERAP1 (Hap8 and Hap10) toward l-AMC at the same or at a double (×2) E:S ratio. When the differences in panels A–D reached statistical significance, this was indicated by their p values.